The skin is a very complex organ that contains different populations of cells,
The outer border of the skin, known as the epidermis, is supported by epidermal stem cells, which are found in the basal layer, while the dermis, the layer below the epidermis, is rich in dermal fibroblasts, which produce extracellular matrix components such as collagen and elastic fibers, which give the skin its elasticity. . Beneath the dermis is subcutaneous fat, which functions as lining, insulation, and energy storage.
However, in the modern world, where many factors and mechanisms influence the quality and health of skin, many of the normal and necessary structures of the skin are missing, so we must find new alternative cells and functional models for the treatment of skin diseases and regeneration.
3D cell organoids contain a variety of cell types that can be used to model organogenesis and developmental disorders. Skin organoids are derived from embryos and can self-organize to form an organized, skin-like structure composed of skin progenitor cells.
The generation of dermal organoids represents a new hope for skin regeneration and is expected to provide a new paradigm for the diagnosis and treatment of skin diseases. Skin organoids can be used to modify physiological functions, such as the sensitivity of cutaneous nerves and the interaction of the microbiome and the skin, as well as to treat cutaneous viral-bacterial infections and lesions of various etiologies.
Advantages of 3D human skin organoids
The use of 3D skin organoids provides a number of advantages:
Similarity to natural skin composition: 3D cultures closely mimic the composition and architecture of human skin, providing a more realistic model.
Complexity of the skin. Incorporation of multiple cell types, growth factors, and extracellular matrix (ECM) components helps recreate skin complexity
More realistic environment: They create a more realistic cellular environment that closely mimics the natural structure and function of human skin.
This allows a more accurate approach to the biology and pathology of the skin.
Increased Physiological Relevance: The three-dimensional nature of 3D skin organoids allows for better representation of intercellular interactions and cell-to-cell interactions, which are critical to understanding the mechanisms of skin diseases and conditions.
Increased predictability and reproducibility: They offer a more reliable and reproducible platform, ensuring that the results obtained are more predictable
These advantages make 3D skin organoids a powerful tool in the treatment of dermatological diseases.
Why do we have resistant cases?
We come across patient cases in which stem cell treatment does not cause any improvement. That is, stem cells do not integrate into damaged cells, taking into account immune resistance to treatment. Also, quite often, in such cases, we are inclined to believe that the diagnosis was not made correctly, that is, the cause (nature) of the disease and the selection of cellular mechanisms to influence the problem were determined.
We will give 3 examples of such patients who did not improve after initial or repeated stem cell therapy. Therefore, it was decided to use therapy with precursor cells, which are easily integrated into cells without causing an active impact of the immune system + clearly correcting the biochemical and morphological processes of inflammation.
Progenitor stem cells therapy, example:
- Exosomes isolated from embryo stem cells .Exosome therapy (eg, exosomes from MSCs)
- Mitochondrial complex: Preparation with mitochondrial complexes (isolated from mesenchymal or embryonic cells with activation of coenzyme Q10 and L-carnitine)
- Ribosomes: Preparations containing ribosomes (ribosome extracts from mesenchymal or embryonic stem cells)
- Tissue metalloproteinase inhibitors (TIMPS): TIMPS reduce the activity of metalloproteinases, preventing the destruction of collagen and the extracellular matrix. natural inhibitors isolated from embryonic stem cells)
- Endothelial stem cells (for vascular damage)
